The comic stars two girls (possibly Tabitha and Lisa) and its theme revolves around girls being superior to guys. When Greg Heffley turns in his comics, he notes the strip in the pile of comics from other kids who are trying out for the job. He says that he isn't too worried, as most of the comics are pretty bad.
A boy named Tyler Green walks near Tabitha and Lisa's table, which results in the two chastising him and telling him he isn't cute. He then falls down, and the girls laugh and say "Girls rule!"
In the second comic, Tabitha and Lisa are sitting near the school wall and meet with Amanda who asks if she can sit with them. The girls reject because she didn't have a phone and wore the same earrings from the day before. Just then a ball hits Amanda and two boys come and tell her to throw their ball back at them. One of the girls gets up and throws the ball at the boy's stomach, telling him to call Amanda by her real name. The three of them hold hands together and say "Girls Rule!".
- Girls Rule! is one of the school comics whose creators are known.